Glycerol stored culture supernatants

Dr Engelbert Buxbaum engelbert_buxbaum at
Thu Jun 17 03:59:38 EST 2004

Lou Roddam wrote:

> Hi,
> I am doing ELISA analysis of monoclonal culture supernatants that have been
> stored in 50% glycerol. The glycerol is interfering with the ELISA but I do
> not want to dilute my supernatants too much. How can I get around this???
> Would incubating at a higher temperature help?

That depends on how many samples you have. For reasonably small numbers,
use gelfiltration to remove the glycerol. 1 ml spin columns should do
the job.

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