Glycerol stored culture supernatants

[Dr Engelbert Buxbaum]

Lou Roddam wrote:

> Hi,
> 
> I am doing ELISA analysis of monoclonal culture supernatants that have been
> stored in 50% glycerol. The glycerol is interfering with the ELISA but I do
> not want to dilute my supernatants too much. How can I get around this???
> Would incubating at a higher temperature help?

That depends on how many samples you have. For reasonably small numbers,
use gelfiltration to remove the glycerol. 1 ml spin columns should do
the job.