PCR GC rich genomic region

Duncan Clark me at privacy.net
Tue Jun 22 03:10:02 EST 2004


Historians believe that in newspost 
<20040622072629.32765.qmail at ww02.hostica.com> on Tue, 22 Jun 2004, 
jinglanl at stanford.edu penned the following literary masterpiece:
>Any suggestion?

Try adding some DMSO maybe up to 10% final.

Try using a Taq:proof-reading pol enzyme mix

Reduce dNTPs to 0.2mM final from 0.4mM

As you have sequence of the 2 bands you do not want can you pick an RE 
that will cut those but not your target?

Duncan
-- 
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.



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