FW: Ethanol in my RNA/TE buffer

Deanne Bell dbell at fresno.ars.usda.gov
Wed Jun 23 15:03:59 EST 2004


Adding ethanol (like 250% of the buffer volume) makes nucleic acids
precipitate out of buffered solution - right?  
A LOT of left over ethanol will prevent nucleic acids from fully
dissolving back into the buffer.  A LITTLE  will probably evaporate out
of the TE solution.

TTFN
Dee Bell



-----Original Message-----
From: owner-methods at hgmp.mrc.ac.uk [mailto:owner-methods at hgmp.mrc.ac.uk]
On Behalf Of Mark Ledesma 
Subject: Ethanol in my RNA/TE buffer
I know it is customary when washing an RNA pellet with 75% ethanol to
dry the ethanol off the pellet before suspending it in TE buffer.
Could someone kindly explain why?

Someone I know who looks kind of like me (it isn't me, really) just
forgot to dry his pellet and surely has some ethanol in with his RNA
and TE. How bad is this?

Thanks for any help,
Mark Ledesma
---



More information about the Methods mailing list