PCR Amplifying 2-5Kb fragments

Phil McClean mcclean at cableone.net
Wed Jun 23 20:54:20 EST 2004

 Does anyone have a procedure in which they can routinely amplify via PCR
fragment 2 to 5 kb in length.  What are some of the critical factors that
you have encountered.  I would rather use standard Tag polymerase.  Don't
have the budget for the more expensive specialized Tag polymerases.

Phil McClean

More information about the Methods mailing list