PCR GC rich genomic region

Serg serg at fidelitysystems.com
Sun Jun 27 20:45:13 EST 2004


Hi,

You can try TopoTaq Pol. It is stable at 97 degree and has strand
displacement activity.

http://www.fidelitysystems.com/TopoTaq.html
http://www.pnas.org/cgi/content/full/99/21/13510
http://www.horizonpress.com/hsp/books/f/fdna.html

serg 

jinglanl at stanford.edu wrote in message news:<20040622072629.32765.qmail at ww02.hostica.com>...
> 
>Do I have to use 
>some special Taq Pol for GC rich region? 
>Do I have to reduce 97 degree denature time 
>to less than 10 seconds? 
> 
> Any suggestion?
> 
> Thanks a lot!
> 
> Jinglan Liu
> 
> http://biowww.net/mynews/tree.php?group_name=bionet_molbio_methds-reagnts&begin=0



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