Betaine

Vidhya Narayanan gtg066j at mail.gatech.edu
Thu Mar 4 21:12:53 EST 2004


Hello!! 
 I am trying to amplify GC rich region using PCR. I have tried using betaine to 
improve the efficiency( 0M to 3.6M). But I am unable to find the optimum 
conditions( annealing and extension temp). I am just not able to get any PCR 
products.It would be great if someone could help me out with this!1

cheers
Vidhya

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