over expression clone

newgene newgene at bigfoot.com
Tue Mar 9 11:11:46 EST 2004


Hi everyone, I wanted to establish a over expression clone by
transfected a gene to the cell line. Finally I got some stable clones
after the drug selection. Surprisingly, I could not obtain any
over-expressors. The gene expression level is even lower than the
endogenous level comparing to the parental cells. Could anyone give an
explanation for the phenomenon?

Thanks

Limei



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