how to concentrate Co-IP protein sample

Wolfgang Schechinger hubahopp at gmx.de
Mon Mar 15 11:44:50 EST 2004


if you just need to see it in the gel, why not simply use ammonium sulfate?

Wo

At 10:50 15.03.2004 -0500, tracy wrote:
>Hi,
>
>I am trying to do a co-IP experiment on a HA tagged protein, and I eluted
>the protein with SDS buffer.  However, the protein level is very low that
>after I ran the eluate on a gel followed by silver staining, I couldn't see
>any band.  So now I am thinking combining all the eluate from multiple
>experiments.  Is there a way that I can concentrate all these proteins?
>Will TCA precipitation work in the presence of SDS?  Or is there any kind of
>concentrator that I can use?  Thank you very much!
>
>
>
>
>
Wolfgang Schechinger


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