how to concentrate Co-IP protein sample

Linan Chen lichen at bsd.uchicago.edu
Mon Mar 15 12:26:52 EST 2004


hi,

UPPA Protein Concentration kit is very good. RC kit from bio-rad uses the 
same reagent(UPPA).

http://www.genotech.com/ProdDisplay.aspx?catid=11

linan
At 09:50 AM 3/15/2004, you wrote:
>Hi,
>
>I am trying to do a co-IP experiment on a HA tagged protein, and I eluted
>the protein with SDS buffer.  However, the protein level is very low that
>after I ran the eluate on a gel followed by silver staining, I couldn't see
>any band.  So now I am thinking combining all the eluate from multiple
>experiments.  Is there a way that I can concentrate all these proteins?
>Will TCA precipitation work in the presence of SDS?  Or is there any kind of
>concentrator that I can use?  Thank you very much!

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