non-coding RNA, cap structure and polyA tail

Domo domo11711 at yahoo.de
Tue Mar 23 01:02:07 EST 2004


Hallo,

1. Do all non-coding RNA (I mean non mRNA) does have 5'-end cap structure?

I am asking, because I want amplify full-length ncRNAs based on current protocols...

2. What's about polyA end? - Are there reports that ncRNA does not have polyA tail?

In this case I would use random priming instead of polyT primer.

Thank you,

Domo



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