RAPD PCR breakdown

[DrAnnie]

Some info that might help:

1)  All of my primers are from Operon and are 10 mers.

2)  Some are older than others but even the fresh ones aren't working.

3)  I have over 100 primers, so I'm not going to list the sequences.

4)  I've used the same MJ Research PTC-200 this whole time. 

5)  After an intial 4 cycles that include a ramp down to a 37 degree
anneal, then there are typical 35 cycles with 94 degree melt, 37
degree anneal, and 72 degree extend with an additional 10 minute
extend at the end after all of the cycles are complete.

6)  Used the same 96 well plates and seals.  Tried to use the same
reagents, but companies have been sold and bought since I started
(including Operon) and so the reagents come with a different name, but
I am assuming the contents are the same as listed.

7)  Have tried fresh dNTP's, Taq, templates, etc. to no avail.

8)  Am about to try a run with snap freezing the primers.

Is there anything else I could tell you that would help?