RACE

juliasung at hotmail.com juliasung at hotmail.com
Tue Mar 23 14:17:01 EST 2004


I have performed rapid amplification of cDNA ends (RACE) on a gene (the sequence of which is only partially known) in bacteria.  Both nested and non-nested RACE products gave almost similar size products respectively.  However, the sequencing results indicate that both fragments are ribosomal RNA sequences (which is not what I am expecting).  I was wondering if anyone may have a possible explanation as to why this happens. 
Many thanks in advance.

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