RAPD PCR breakdown
amyezers at kings.edu
Wed Mar 24 09:41:54 EST 2004
Thanks for some input.
I just got the thermal cycler back from service where they replaced
the block -- didn't help.
We aren't using a hot start Taq (maybe we should?). I ordered new Taq
to replace the quesitonable one. But the Taq which was originally
Gibco is now sold by Invitrogen. Can anybody suggest the best Taq for
We have a 16S bacterial amplification that works beautifully on the
same machine but both our RAPD amplifications for beetles and fish are
We've just tried snap-freezing the primers, but I don't have those
results yet. We do dilute those in water.
Thanks for the tip on the stored DNA -- haven't tried that yet.
Any other suggestions are welcome!
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