siRNA transfection into MCF-7 breast cancer cells

Peter Frank peter_frankde at yahoo.de
Thu Mar 25 04:54:09 EST 2004


scimedweb at mail.com (scimedweb) wrote in message news:<7481f2c9.0403230419.11159434 at posting.google.com>...
> Dear all,
> 
> What is the most efficient method to transfect siRNA into MCF-7 cells.
> It is usually difficult to transfect these cells except with calcium
> phosphate.

In my experience lipofection works best for transfecting siRNA into
mammalian cells. There are several lipofection kits available from
different companies. I have only worked with two of them, so I can't
tell you which one is best.

Peter



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