Hogness Modified Freezing Medium (HMFM)
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Fri Mar 26 18:23:45 EST 2004
In article <c40rsi$lm8$1 at south.jnrs.ja.net>, "Raymond Dalgleish" <ray at le.ac.uk> wrote:
>Over twenty years ago when I was a post-doc at the NIH, somebody gave me a
>recipe for an excellent freezing medium for E. coli. You just add a one
>tenth volume to the broth either before or after growing the cells. It's
>called Hogness Modified Freezing Medium.
>Does anybody know what the original source of this stuff is? Is it published
10X Hogness Modified Freezing Medium:
Solution A: 5 mM MgSO4x4 H2O, 20 mM Sodium Citrate,
85 mM (NH4)2SO4, 45 % Glycerin.
Solution B: 0,66 M KH2PO4, 1,3 M K2HPO4
Dilute "B" 4X with "A" = 10 x HMFM (e.g., 1B + 4A)
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