Some questions about Polyacrylamide gels

Fabio fabiodb at katamail.com
Sat Mar 27 04:37:23 EST 2004


Il Sun, 07 Mar 2004 21:27:47 +0000, Ferdinando Pucci ha scritto:

> On Sun, 07 Mar 2004 15:58:11 +0100
> Fabius <FabioDB#deleteme#@katamail.com> wrote:
> 
>> this methods don't work, but in that case why don't work?
> 
> I've never done a PAGE, just studied, but an agarose basement isn't useful to
> pour a PAGE cos it's open to the air: O2 inhibits the crosslinking of
> acrilamide.

I supose that I can "cover" the gel with butanol to resolve this problem.

Thanks

--Fabio




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