Some questions about Polyacrylamide gels
David L Haviland
David.L.Haviland at uth.tmc.edu
Sat Mar 27 10:49:18 EST 2004
Just my $0.02 worth, we had people in the lab that were sensitive to
butanol, so in a pinch until these people left the lab, we simply use
distilled water to overlay the gels. More care must be taken with
water than butanol as the water, if forcefully added to the gel, can
dilute the top part. But if applied with care, it works just as well.
David L. Haviland, Ph.D., Asst. Prof. Immunology
University of Texas - Houston, H.S.C.
Institute of Molecular Medicine, R907
2121 W. Holcombe Blvd., Houston, TX 77030
If everything seems to be going so well, you have obviously
----- Original Message -----
From: "Fabio" <fabiodb at katamail.com>
Date: Saturday, March 27, 2004 3:37 am
Subject: Re: Some questions about Polyacrylamide gels
> Il Sun, 07 Mar 2004 21:27:47 +0000, Ferdinando Pucci ha scritto:
> > On Sun, 07 Mar 2004 15:58:11 +0100
> > Fabius <FabioDBemail@example.com> wrote:
> >> this methods don't work, but in that case why don't work?
> > I've never done a PAGE, just studied, but an agarose basement
> isn't useful to
> > pour a PAGE cos it's open to the air: O2 inhibits the
> crosslinking of
> > acrilamide.
> I supose that I can "cover" the gel with butanol to resolve this
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