ELISA with cell lysates?

Dr Engelbert Buxbaum engelbert_buxbaum at hotmail.com
Sun Mar 28 02:39:48 EST 2004

Peter Frank wrote:

> Does anyone have any experience with the use of ELISAs in combination
> with cell lysates? Or can anyone give me any references where ELISAs
> have been used for quantitating proteins in cell lysates?

Have you considered doing dot blots instead? That way you can remove any
interference and quantitation is much more reliable.

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