Cell culture synchronisation methods?

Peter Frank peter_frankde at yahoo.de
Mon Mar 29 10:16:42 EST 2004


Hello,

Does anyone here have experience with cell culture synchronisation? I
have read about serum starvation and use of lovastatin or aphidicolin
as reversible inhibitors of cell cycle progression.

Which cell synchronisation method would you recommend? Do cell
synchronisation methods put a lot of stress on cell cultures? Would be
this be a problem for subsequent gene expression studies?

Is cell synchronisation necessary when freshly split cultures or
cultures revived from DMSO cell stocks of less than a week's age are
used for the gene expression experiments?

Peter



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