Low OD using Trizol to isolate total RNA from cell lines

Alex chen.1069 at osu.edu
Sun May 2 23:42:34 EST 2004


Hi all,

I use Trizol to isolate total RNA from mouse cell line. Sometimes I
get a very poor 260/280 ratio (0.9-1.2).  I think it might be due to
protein contamination. Has anyone there ever tried any modification to
this protocol to get a good yield and better 260/280 ratio? What
method do you use to purify the total RNA after you check with a
spectrophotometer( besides phenol:chloroform extraction from Maniatis
)?

Thanks,

Alex



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