Biorad Bradford Assay

OK oy_khan at yahoo.com
Fri May 7 17:30:03 EST 2004


I am using a Biorad Bradford Protein Assay to estimate total protein from
tissue lysates, fir westrn blot analysis. I use a dilution of 1:20 to 1:50
of the lysates to estimate protein. At this dilution I should not get any
interference in the assay from the SDS, NP40, and Na-Deoxycholate (present
in the tissue lysis buffer). However the estimation are not always
reproducible.
Does anyone have any tips/advice on this issue, please

Thanks

OK





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