Biorad Bradford Assay

D.K. dk at
Fri May 7 19:32:14 EST 2004

In article <5e98ae49e1b7861e50ade6924f5ab4c5 at>, "OK" <oy_khan at> wrote:
>I am using a Biorad Bradford Protein Assay to estimate total protein from
>tissue lysates, fir westrn blot analysis. I use a dilution of 1:20 to 1:50
>of the lysates to estimate protein. At this dilution I should not get any
>interference in the assay from the SDS, NP40, and Na-Deoxycholate (present
>in the tissue lysis buffer). However the estimation are not always
>Does anyone have any tips/advice on this issue, please

1) Work more carefully, be more accurate at all steps.
2) Try to take the spec reading at the same amount of time after 
mixing protein into the reagent. About 10-20 min is optimal.
The absorbance does changes with time.


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