Krebs buffer cloudy

Wolfgang Schechinger hubahopp at gmx.de
Wed May 12 16:42:39 EST 2004


Maybe it's a pH problem that makes the Ca precipitate. 

See http://www2.swmed.edu/rogersmr/perfusates.htm 
http://www2.swmed.edu/rogersmr/perfusates.htm#Krebs-Henseleit%20(KH)%20Bicar
bonate%20Buffer
for details. 

Just was googling for "krebs buffer cloudy" ...

HTH

Wo

At 08:01 12.05.2004 -0700, S.E. wrote:
>I am making up Krebs buffer for an isolated-perfused heart
>preparation.  The recipe for the buffer is as follows: NaCl 118mM,
>NaHCO3 25mM, KCl 4.8mM, KH2PO4 1.2mM, MgSO4 1.2mM, Glucose 11mM
>CaCl2(2H2O) 1.5mM and Sodium Pyruvate.
>
>I was told to dissolve the chemicals in order and make sure that each
>item is dissolved completely before I add the next chemical or else
>the buffer will become cloudy.  Unfortunately, even if I follow those
>directions, the buffer will still become cloudy as soon as I add the
>CaCl2 quite frequently.
>
>Another researcher suggested to bubble the solution with 95%CO2/5%O2
>for 5 minutes before adding the CaCl2, but that didn't solve the
>problem.
>
>I'd really like to be able to be able make to make this buffer
>regularly without having to throw out the first 3 preparations because
>they cloud up as soon as add the CaCl.....
>
>Any suggestions?=20
>
>Thanks is advance,=20
>Stephanie
>
>
Wolfgang Schechinger


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