how to isolate membrane fraction from cells

brenda b.hendriks at wkz.azu.nl
Sat May 15 04:00:49 EST 2004


Hi there,

For my project I need to make clean Westren blots. The only way I have not
tried yet is to purify the membrane fraction from my cells (BAF/B03, which
is a murine precursor B cell line). The protein I am looking for is the
human growth hormone receptor, which is a membrane bound protein.
Can somebody please tell me how to isolate this membrane fraction? I have
heard about specialised kits (but without references) and some techniques
using high speed centrifugation.
Please help me out.

Thanks a lot,
Brenda





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