Krebs buffer cloudy

Dr Engelbert Buxbaum engelbert_buxbaum at hotmail.com
Thu May 20 12:49:53 EST 2004


S.E. wrote:

> I am making up Krebs buffer for an isolated-perfused heart
> preparation.  The recipe for the buffer is as follows: NaCl 118mM,
> NaHCO3 25mM, KCl 4.8mM, KH2PO4 1.2mM, MgSO4 1.2mM, Glucose 11mM
> CaCl2(2H2O) 1.5mM and Sodium Pyruvate.

Dissolve the CaCl2 seperately in half the volume of water, than add to
the solution of the other chemicals under vigorous stirring. Ca3(PO4)2
is only slightly soluble in water, you must not exceed the solubility
product as it will not redissolve.



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