Why DTT in RT-PCR?

D.K. dk at no.email.thankstospam.net
Sun May 30 13:23:06 EST 2004


On 30 May 2004 09:49:33 -0700, philipp.bassler at gmx.de (Philipp) wrote:

>Hi!
> 
>I have a short question: Why is ist necessaire to use DTT-Solution for
>a RT-PCR? What does it result in?

If it is really necessary then it must be that some enzyme 
(reverse transcriptase) is particularly sensitive to oxidation. 

DK




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