How do I sterilize tiny volumes of solutions?

Siddhartha S Mitra ssmitra at
Tue Nov 2 14:35:43 EST 2004

Paul Wary wrote:
> Hello,
> I have tiny volumes of solutions (around 100 µl) that I need to
> sterilize before use in cell culture. Autoclaving is no option because
> the solvents are not stable enough. Filtration doesn't work with the
> filters we have because they almost completely soak up the entire
> volume during filtration. Are there any special filters for this
> purpose or any other options for sterilizing such tiny volumes?
> Paul

Anybody every tried using the microfuge membranes. I have used it once 
and lost about 10ul

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