why glycerol in lysis buffer?

Siddhartha S Mitra ssmitra at buffalo.edu
Tue Nov 2 14:38:08 EST 2004


D.K. wrote:
> On 1 Nov 2004 12:26:37 -0800, irab7 at aol.com (Ira Bell) wrote:
> 
> 
>>What is is the role of  glycerol in the lysis buffer used for
>>immunoprecipitation (mammalian proteins)?
> 
> 
> To reduce "non-specific" interactions between proteins. 
> 
> DK
> 
HOW?????

I thought glycerol was used to create a density gradient for removing 
lipids. I use glycerol for clearing the cytoplasm from the yolk of 
xenopus oocytes.



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