why glycerol in lysis buffer?
Siddhartha S Mitra
ssmitra at buffalo.edu
Tue Nov 2 14:38:08 EST 2004
D.K. wrote:
> On 1 Nov 2004 12:26:37 -0800, irab7 at aol.com (Ira Bell) wrote:
>
>
>>What is is the role of glycerol in the lysis buffer used for
>>immunoprecipitation (mammalian proteins)?
>
>
> To reduce "non-specific" interactions between proteins.
>
> DK
>
HOW?????
I thought glycerol was used to create a density gradient for removing
lipids. I use glycerol for clearing the cytoplasm from the yolk of
xenopus oocytes.
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