why glycerol in lysis buffer?

Toai T NGUYEN ttnguyen at uci.edu
Tue Nov 2 20:25:00 EST 2004


Siddhartha S Mitra wrote:
> D.K. wrote:
> 
>> On 1 Nov 2004 12:26:37 -0800, irab7 at aol.com (Ira Bell) wrote:
>>
>>
>>> What is is the role of  glycerol in the lysis buffer used for
>>> immunoprecipitation (mammalian proteins)?
>>
>>
>>
>> To reduce "non-specific" interactions between proteins.
>> DK
>>
> HOW?????
> 
> I thought glycerol was used to create a density gradient for removing 
> lipids. I use glycerol for clearing the cytoplasm from the yolk of 
> xenopus oocytes.

Protein people, myself included, usually include 10% glycerol in the lysis 
buffer as cryo-protectant, so they can freeze the intermediate lysates and 
purified materials at -80ºC without denaturing the proteins of interest.



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