why glycerol in lysis buffer?
Toai T NGUYEN
ttnguyen at uci.edu
Tue Nov 2 20:25:00 EST 2004
Siddhartha S Mitra wrote:
> D.K. wrote:
>> On 1 Nov 2004 12:26:37 -0800, irab7 at aol.com (Ira Bell) wrote:
>>> What is is the role of glycerol in the lysis buffer used for
>>> immunoprecipitation (mammalian proteins)?
>> To reduce "non-specific" interactions between proteins.
> I thought glycerol was used to create a density gradient for removing
> lipids. I use glycerol for clearing the cytoplasm from the yolk of
> xenopus oocytes.
Protein people, myself included, usually include 10% glycerol in the lysis
buffer as cryo-protectant, so they can freeze the intermediate lysates and
purified materials at -80ºC without denaturing the proteins of interest.
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