Cloning methylated DNA
nospam at nspam.com
Wed Nov 3 12:06:45 EST 2004
> hi everyone
> my lab mostly use DH5alpha competent cells for transformation. however,
> currently, i want to clone methylated dna. i read papers and most of the
> people use XL1-blue competent cells. I am just wondering if it is possible
> DH5alpha and i guess probably not. Am I right? Thanks!
Are you asking if DNA that is methylated and ligated to a vector
can be used to transform DH5a? If so, I see no reason why not,
(although there may be one). Most of the DNA that I prepare from
various strains is assumed to be methylated, and I have routinely
used DH5a as the recipient strain for my cloning procedures.
I guess you could be asking whether DH5a will maintain the
methylation pattern. I think it probably will, as it is neither
dam nor dcm minus (as far as I can recall).
Hope that is in someway useful.
More information about the Methods