About TE and Tris-HCl sterilize with autoclave

Kyle Legate legatek at hotmail.com
Wed Nov 3 14:32:25 EST 2004


Ned Mantei wrote:
> In article <vuybreh8v83.fsf at soggy-fibers.csail.mit.edu>,
> Tom Knight <tk at csail.mit.edu> wrote:
>
>> fabio <fabio at fabius.it> writes:
>>> I want know if a cycle of 120°C for 20'-30' in autoclave can damage
>>> the TE and TRis-HCl solutions.
>>
>> No, but a typical autoclave might leave them less pure than you
>> might like. If I cared about that, I'd filter sterilize.
>
> Filter sterilizing will not inactivate, e.g., RNase.
>
Neither will autoclaving. Baking is required to kill RNAse. 





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