Cell-surface molecule control for FACS

Dr Engelbert Buxbaum engelbert_buxbaum at hotmail.com
Wed Nov 10 09:46:23 EST 2004


Ian A. York wrote:

> I'm looking for a molecule as a control for flow cytometry.  What I need 
> is a protein with the following characteristics:
>  o  Expressed on many (human) tissue types and cell lines
>  o  Expressed at relatively high levels, so easily detected
>  o  Expressed at relatively constant levels (i.e. minimally sensitive to 
> cell cycle stage or stress)
>  o  Antibodies easily available
>  o  NOT MHC class I, since that's what I'm trying to control for.

Housekeeping proteins like Na/K-ATPase, Na/glucose-symporter or the
like?



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