Preparation of electrocompetent cells

Stein Tore Solem steins at nfh.uit.no
Wed Nov 17 06:11:30 EST 2004


Hello everybody

I find it difficult to obtain high transformation efficiencies (>10^9cfu/µg)
using electrocompetent cells (E. coli - TG1) prepared by the standard
protocols (Sambrook & Russel/Current protocol...).

What is the pitfall of the standard protocol? How can I easily optimise the
efficiency?

Alternatively:  Does anybody know an alternative, "foolproof", method that
really gives highly competent cells?


Stein Tore Solem




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