Preparation of electrocompetent cells

[Stein Tore Solem]

Hello everybody

I find it difficult to obtain high transformation efficiencies (>10^9cfu/µg)
using electrocompetent cells (E. coli - TG1) prepared by the standard
protocols (Sambrook & Russel/Current protocol...).

What is the pitfall of the standard protocol? How can I easily optimise the
efficiency?

Alternatively:  Does anybody know an alternative, "foolproof", method that
really gives highly competent cells?


Stein Tore Solem