about sequencing and labels
Nina Mononen
nina.mononen at uta.fi
Tue Nov 23 07:53:03 EST 2004
Can anything be done with following:
In TaqMan assay (for SNP genotyping) I have unlabelled PRC primers and
two oligos labelled with VIC anf FAM that hybridise to the PCR product
born in the amplification using this assay mix. If I take some of this
PCR product and use it to make sequencing reaction using BigDye 3, do
the unincorporated labelled oligos cause problems in sequencing because
of the VIC and FAM dyes? Or what about the hybridised oligos?
Nina
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