about sequencing and labels
blackhole at abuse.plus.com
Tue Nov 23 10:08:40 EST 2004
Historians believe that in newspost <41A332AF.2010307 at uta.fi> on Tue, 23
Nov 2004, Nina Mononen <nina.mononen at uta.fi> penned the following
>If I take some of this PCR product and use it to make sequencing
>reaction using BigDye 3, do the unincorporated labelled oligos cause
>problems in sequencing because of the VIC and FAM dyes? Or what about
>the hybridised oligos?
Use a PCR clean up kit on your PCR product or an ExoSAP reaction that
will destroy dNTPs and primers.
or similar from elsewhere.
I love deadlines. I especially like the whooshing noise they make as
they go flying by.
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