Taqman SNP assay

Nina Mononen nina.mononen at uta.fi
Mon Oct 4 01:49:35 EST 2004


While working with my first TaqMan assay I accidentally added 0.625 ul 
of the assay mix to each reaction (instead of the 1.25 ul) and the assay 
worked fine : ) So, was I just lucky? Do these assays generally work 
with less amount of assay mix added than what is recommended on the 
Applied Biosystems instructions?

Tampere, Finland

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