Taqman SNP assay

Duncan Clark blackhole at abuse.plus.com
Mon Oct 4 04:33:33 EST 2004


Historians believe that in newspost <4160F27F.2050704 at uta.fi> on Mon, 4 
Oct 2004, Nina Mononen <nina.mononen at uta.fi> penned the following 
literary masterpiece:
>While working with my first TaqMan assay I accidentally added 0.625 ul 
>of the assay mix to each reaction (instead of the 1.25 ul) and the 
>assay worked fine : ) So, was I just lucky? Do these assays generally 
>work with less amount of assay mix added than what is recommended on 
>the Applied Biosystems instructions?

You need to define what you mean by assay mix?

You can't be meaning, with that volume, the mastermix so I'm guessing 
you mean amount of primer probe mix?

Duncan
-- 
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.



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