Taqman SNP assay
nina.mononen at uta.fi
Mon Oct 4 05:48:41 EST 2004
Assay mix is the one that has PCR primers and probes, master mix
contains enzymes etc (at least according to the instruction leaflets
that come with the assays : )
Duncan Clark wrote:
> Historians believe that in newspost <4160F27F.2050704 at uta.fi> on Mon,
> 4 Oct 2004, Nina Mononen <nina.mononen at uta.fi> penned the following
> literary masterpiece:
>> While working with my first TaqMan assay I accidentally added 0.625
>> ul of the assay mix to each reaction (instead of the 1.25 ul) and the
>> assay worked fine : ) So, was I just lucky? Do these assays generally
>> work with less amount of assay mix added than what is recommended on
>> the Applied Biosystems instructions?
> You need to define what you mean by assay mix?
> You can't be meaning, with that volume, the mastermix so I'm guessing
> you mean amount of primer probe mix?
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