cloning problem for expression vector

shazdefaz at shazdefaz at
Tue Oct 5 20:45:05 EST 2004


i'm working on a plasmid pMG36e and pNZ8048 plasmid vectors for lactococcus lactis. I'm trying to clone an insert into them but to no avail.

generally i clone the insert and transform into e.coli (as done in revision review) to amplified the plasmid but no result. 
i've check the RE used for digestion and it is OK.
and i've tried a lot of ligase from different product but no result.

can anyone please help me with this? i'm already at it for months now.

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