adding non-palindromic r.e site to primer

arasu a_thiruna at
Fri Oct 8 06:30:25 EST 2004

Hi, i dont know whether i am missing something here- if non
palindromic  r.e site is added to both forward and reverse primers
(both have the same additional nucleotides at the 5' end), in the
final ligation will there be a problem- i was trying for enzyme
bsu361, when i was writing out the sites and aligning the ligation on
paper- one base pair was not aligning-will it be repaired in the
bacterial host or am i missing something.

in practice, it might not be a problem but i am wondering whether it
is the case in theory too!

thanks a lot


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