DNA sequence/ligation problems

Markus Winter m.winter at auckland.ac.nz
Wed Oct 20 11:02:58 EST 2004


> I have a digested insert and vector. Both have an AflII restriction site so i
> can ligate them together. Ligation seems to work, I transform colonies, and
> midi-prep the plasmid. Sequencing results show a 24 basepair sequence in
> between my fusion gene and my target gene. Can anyone help?

Can you give a bit more info (e.g. the sequenced bit).

Markus




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