yeast growth

David Sweetman sweetmanda at cf.ac.uk
Fri Oct 22 02:38:06 EST 2004


Jo,

There are reports in the literature of reduced interacting efficiency when
strains are not maintained on stringent media. Which Y2H reporters are you
using? If you are using the 4 reporter system, are you also selecting for
Adenine?

I wonder if you have selected for a yeast strain carrying fewer copies of
your plasmids and therefore not able to synthesise the same levels of
Histidine as your transformed yeast?

Atb

David



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