yeast growth

Maple J jm151 at le.ac.uk
Mon Oct 25 11:02:57 EST 2004


I hadn't thought along those lines, but I doubt that my proteins could have
an affect as they are involved in a division pathway in plants.

thank you for your time

jo


"Rafael Maldonado" <not-this at not-this.com> wrote in message
news:cl83f2$h0s$1 at news.ua.es...
> Maple J wrote:
> > we have been using the yeast two hybrid system to analyse
protein-protein
> > interactions.  using the histidine reporter gene we have found that some
of
> > our strains containing interacting proteins can grow more on media
lacking
> > histidine than media containing histidine and cannot think of a reason
for
> > this.  can anyone please offer any suggestions?
> >
> > thank you for your time in advance
> >
> > jo
> >
> >
> Maybe your protein is related to the regulation of histidine biosynthesis?
>
> -- 
> Rafael Maldonado, Ph. D.
> Division of Genetics
> University of Alicante
> Spain





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