stable transfection of Huh7 and Hep3B

Maggie hydai at
Wed Oct 27 04:10:21 EST 2004

Dear listers,
Does anyone have experience in transfection of HCC cell lines,eg. Huh7
and Hep3B? I have tried some lipid-mediated transfer method,
eg.lipofectamine 2000 (invitrogen) and Fugene6(Roche), but it doesn't
work. Any suggestion will be appreciated.


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