How do I sterilize tiny volumes of solutions?

Paul Wary paul_wary at
Fri Oct 29 09:48:34 EST 2004


I have tiny volumes of solutions (around 100 µl) that I need to
sterilize before use in cell culture. Autoclaving is no option because
the solvents are not stable enough. Filtration doesn't work with the
filters we have because they almost completely soak up the entire
volume during filtration. Are there any special filters for this
purpose or any other options for sterilizing such tiny volumes?


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