How do I sterilize tiny volumes of solutions?
dk at no.email.thankstospam.net
Fri Oct 29 22:00:11 EST 2004
On 29 Oct 2004 07:48:34 -0700, paul_wary at yahoo.com (Paul Wary) wrote:
>I have tiny volumes of solutions (around 100 µl) that I need to
>sterilize before use in cell culture. Autoclaving is no option because
>the solvents are not stable enough. Filtration doesn't work with the
>filters we have because they almost completely soak up the entire
>volume during filtration. Are there any special filters for this
>purpose or any other options for sterilizing such tiny volumes?
There are filters with retention of only 10 microL I use them regularly
for analytical HPLC and occasionally for sterilization.
This is catalog # for 0.2 u filters
but the picture is *wrong*. The filters in reality look like this:
(you can buy these and sterilize them with ethylene oxyde
yourself - this is what Millipore does anyway).
More information about the Methods