Dot blotting lipids

Mad Mat hauwelm at minitel.net
Thu Sep 9 03:45:25 EST 2004


Dear all,
I want to do a dot blot with lipids (phosphoinositides), what kind of
membrane shall I use ? I am also wondering whether the conventional
cell lysate preparation for Western blot will work (1% triton X100) or
do I need to use very stringent detergents at high concentration ?

Thanks

MM



More information about the Methods mailing list