Dot blotting lipids

Mad Mat hauwelm at
Thu Sep 9 03:45:25 EST 2004

Dear all,
I want to do a dot blot with lipids (phosphoinositides), what kind of
membrane shall I use ? I am also wondering whether the conventional
cell lysate preparation for Western blot will work (1% triton X100) or
do I need to use very stringent detergents at high concentration ?



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