Automatic DNA Seqencer - which one is better?
j131 at op.dot.pl
Fri Sep 10 02:11:33 EST 2004
In article <29f2499d.0409090415.e348395 at posting.google.com>, bounty at poczta.fm (Anna) wrote:
>zachnilsen at yahoo.com (Zach) wrote in message
> news:<17bb234a.0409081709.39844cab at posting.google.com>...
>> We have the ABI 377 sequencer and it's a pain to use. I would not
>> recommend this outdated technology to anyone.
>> Shortly, I'm going to use the 16 capillary ABI 3100 that is available
>> in the lab across the hall. Fellow students say that it requires
>> minimal effort to get great sequences without going through the pain
>> to cast gels and load samples manually. There is no well-to-well
>> leakage, because each sample runs in a separate capillary.
>I have a little problem...it costs a fortune... What do you think
>about ABI PRISM 310? Is that very bad? and old? Do you know any more
>sequencers? More automated of course.
Dont buy 310 unless it is not sequencing you are interested in.
Short reads, one capillary short life of laser: waste of time and money.
And I dont think you can buy 377 anymore.
I am afraid there is really no "cheap and reliable" automatic sequencer.
If you cant afford 3100 then you would be much better off buing sequencing
reactions and not the sequencer.
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