Designing degenerate primers

Manuel Tomé mctome at ci.uc.pt
Fri Sep 10 11:22:42 EST 2004


Greetings!
A newbie question: after doing a multiple protein (or cDNA) alignment 
on the program I usually use, I am not able to save the consensus 
sequence. Therefore it is very tedious to enter it manually to design 
degenerate primers.
I am sure it must be easy to do this with some kind of application 
somewhere out there! Make a multiple alignment, save the consensus 
sequence and directly use it for primer design! Or am I missing 
something obvious?

Manuel Tome'

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