DNA fragment isolation using DEAE membranes
nielsen at immunbio.mpg.de
Fri Sep 10 11:43:26 EST 2004
Since Schleicher and Schuell stopped making NA45, it has not been clear
where one can obtain DEAE-membranes for the isolation of DNA fragments
from agarose gels.
I have now found a replacement for NA45 which I am very happy with.
It is a DEAE-membrane from the complany Sartorius (www.sartorius.com)
called Sartobind-D (Cat# D210297D).
I have not gotten the chance to extensively test the fragments isolated
with the method but at least restriction digestions, PCR and cloning of
the purified fragments works well and the yields are good.
The membrane is much stiffer than NA45 so it is much easier to work
A protocoll can be found here:
Dr. Peter J. Nielsen
Max-Planck-Institute for Immunobiology
D-79108 Freiburg i.Br.
tel +49 761 5108 319
fax+49 761 5108 221
email: nielsen at immunbio.mpg.de
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